Translational_Unit

Part:BBa_K1639007:Design

Designed by: Mustafa Yılmaz   Group: iGEM15_ATOMS-Turkiye   (2015-09-13)


DAMP-Pexiganan


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 108
    Illegal XhoI site found at 508
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains an additional BamHI restriction site at beginning of DAMP-Pex sequence and a XhoI restriction site between DAMP-Pex and double terminator(B0010-B0012). Also have a TEV Cleaveage site(ENLYFQS) between DAMP and Pex.

To achieve constitutive expression of this protein complex we put lacIq promoter at the beginning. It is a single base changed version of lacI promoter which boosts expression of gene about 10-fold. As this promoter cannot be terminated by T7 terminator like most of our parts we inserted double terminator (B0010-B0012) at the end.

Source

none. Synthetic.

References